constructs encoding pum1 Search Results


constructs encoding pum1  (OriGene)
90
OriGene constructs encoding pum1
Figure 3: <t>PUM1</t> and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.
Constructs Encoding Pum1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/constructs+encoding+pum1/Pumilio+1+(PUM1)+(NM_014676)+Human+Tagged+ORF+Clone/pm30197756-194-0-22
Average 90 stars, based on 1 article reviews
constructs encoding pum1 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Figure 3: PUM1 and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: Figure 3: PUM1 and PUM2 proteins bind and regulate SPIN1 and SPIN3 mRNAs. Schematic of full-length human SPIN1 and SPIN3 3ʹUTRs (A). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black (SPIN3). Short 3ʹUTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3ʹUTRs are indicated in brackets, with position within the 3ʹUTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) (B). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level (C). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting (D). Graphs represent average values with standard errors. P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Negative Control, Knockdown, Isolation, Expressing, Over Expression, Western Blot

Figure 4: Influence of PUM1 and PUM2 proteins on luciferase reporter constructs carrying SPIN1 or SPIN3 3ʹUTRs. The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3ʹUTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) (A). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3ʹUTR, which lacks PBE motifs (negative control) (B). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3ʹUTRs (C). Effects of PUM1 or PUM2 overexpression (D). or knockdown (E). on luciferase constructs containing short SPIN1 or SPIN3 3ʹUTR fragments. **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: Figure 4: Influence of PUM1 and PUM2 proteins on luciferase reporter constructs carrying SPIN1 or SPIN3 3ʹUTRs. The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3ʹUTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) (A). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3ʹUTR, which lacks PBE motifs (negative control) (B). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3ʹUTRs (C). Effects of PUM1 or PUM2 overexpression (D). or knockdown (E). on luciferase constructs containing short SPIN1 or SPIN3 3ʹUTR fragments. **P ≤ 0.005, ***P ≤ 0.0005, ****P ≤ 0.00005.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: Luciferase, Construct, Expressing, Over Expression, Plasmid Preparation, Negative Control, Knockdown

Figure 5: PUM1 induces TCam-2 cell apoptosis. TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs (A). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector (B), PUM1 (C), or PUM2 (D) constructs.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: Figure 5: PUM1 induces TCam-2 cell apoptosis. TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs (A). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector (B), PUM1 (C), or PUM2 (D) constructs.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: Transfection, Construct, Plasmid Preparation, Cell Culture

Figure 6: PUM1 and PUM2 slightly downregulate TCam-2 cell cycle progression. PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: Figure 6: PUM1 and PUM2 slightly downregulate TCam-2 cell cycle progression. PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: