constructs encoding pum1 Search Results


pum1 rc201219  (OriGene)
90
OriGene pum1 rc201219
Pum1 Rc201219, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pum1 rc201219/product/OriGene
Average 90 stars, based on 1 article reviews
pum1 rc201219 - by Bioz Stars, 2026-03
90/100 stars
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constructs encoding pum1  (OriGene)
89
OriGene constructs encoding pum1
Schematic of full-length human SPIN1 and SPIN3 3′UTRs ( A ). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black ( SPIN3 ). Short 3′UTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3′UTRs are indicated in brackets, with position within the 3′UTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in <t>RIP-PUM1</t> and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) ( B ). Influence of <t>PUM1</t> and PUM2 proteins on endogenous SPIN mRNA level ( C ). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting ( D ). Graphs represent average values with standard errors. P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005.
Constructs Encoding Pum1, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/constructs encoding pum1/product/OriGene
Average 89 stars, based on 1 article reviews
constructs encoding pum1 - by Bioz Stars, 2026-03
89/100 stars
  Buy from Supplier

Image Search Results


Schematic of full-length human SPIN1 and SPIN3 3′UTRs ( A ). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black ( SPIN3 ). Short 3′UTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3′UTRs are indicated in brackets, with position within the 3′UTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) ( B ). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level ( C ). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting ( D ). Graphs represent average values with standard errors. P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: Schematic of full-length human SPIN1 and SPIN3 3′UTRs ( A ). PBE-like motifs responsible for PUF-domain binding are in red and UGUA core motifs are in black ( SPIN3 ). Short 3′UTR fragments containing PBE motifs, which were used for luciferase reporter assays, and full-length 3′UTRs are indicated in brackets, with position within the 3′UTR starting from the end of the stop codon. Enrichment of SPIN1 and SPIN3 in RIP-PUM1 and RIP-PUM2 (indicated by RIP P1 and RIP P2, respectively) was measured via RT-qPCR and was compared to the negative control (nonimmune IgG, indicated by RIP IgG) ( B ). Influence of PUM1 and PUM2 proteins on endogenous SPIN mRNA level ( C ). PUM1 and PUM2 siRNA knockdown efficiencies are shown on the left. Total RNA was isolated from TCam-2 cells in the presence of actinomycin D. SPIN expression was measured via RT-qPCR and was compared to that in untransfected cells. PUM1 or PUM2 overexpression downregulated endogenous SPIN1 as measured by western blotting ( D ). Graphs represent average values with standard errors. P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Negative Control, Isolation, Expressing, Over Expression, Western Blot

The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3′UTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) ( A ). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3′UTR, which lacks PBE motifs (negative control) ( B ). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3′UTRs ( C ). Effects of PUM1 or PUM2 overexpression ( D ). or knockdown ( E ). on luciferase constructs containing short SPIN1 or SPIN3 3′UTR fragments. ** P ≤ 0.005, *** P ≤ 0.0005, **** P ≤ 0.00005.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: The effects of PUM proteins on SPIN expression were assessed using a dual luciferase assay. Luciferase reporter constructs carrying full-length 3′UTRs for SPIN1 (upper panel) or SPIN3 (lower panel) were tested with PUM1 (P1) or PUM2 (P2) overexpression or empty pCMV6-entry vector (pC) ( A ). Effects of PUM overexpression on luciferase reporter construct carrying full-length GAPDH mRNA 3′UTR, which lacks PBE motifs (negative control) ( B ). Effects of siRNA-mediated PUM1 (P1 KD) or PUM2 (P2 KD) knockdown (KD) on luciferase reporter constructs carrying full-length SPIN 3′UTRs ( C ). Effects of PUM1 or PUM2 overexpression ( D ). or knockdown ( E ). on luciferase constructs containing short SPIN1 or SPIN3 3′UTR fragments. ** P ≤ 0.005, *** P ≤ 0.0005, **** P ≤ 0.00005.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: Expressing, Luciferase, Construct, Over Expression, Plasmid Preparation, Negative Control

TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs ( A ). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector ( B ), PUM1 ( C ), or PUM2 ( D ) constructs.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: TCam-2 cells were transfected with constructs encoding PUM1 (P1), PUM2 (P2), or empty vector and cultured for 48 h. Apoptosis was measured as described for SPINs ( A ). Dot-plot showing quality of TCam-2 cell separation into living, necrotic, and early or late apoptotic populations after transfection with empty vector ( B ), PUM1 ( C ), or PUM2 ( D ) constructs.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: Transfection, Construct, Plasmid Preparation, Cell Culture

PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

Journal: Oncotarget

Article Title: SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma

doi: 10.18632/oncotarget.25977

Figure Lengend Snippet: PUM1 and PUM2 were separately overexpressed and the effects on cell cycle progression were measured 72 h later.

Article Snippet: Constructs encoding PUM1 (RC201219), PUM2 (RC211307), SPIN1 (RC201938), or SPIN3 (RC215063), in the pCMV6-entry vector system for protein overexpression were purchased from OriGene Technologies.

Techniques: